Publikationen 2020

  • Tröndle J, Schoppel K, Bleidt A, Trachtmann N, Sprenger GA, Weuster-Botz D (2020): Metabolic control analysis of Escherichia coli producing L-tryptophan from glycerol based on data from a parallelized short-term perturbation experiment. J Biotechnol 307: 15-28.

Publikationen 2019

  • Mähler C, Burger C, Kratzl F, Weuster-Botz D, Castiglione K (2019): Asymmetric whole-cell bioreductions of (R)-carvone using optimized ene reductases. Molecules 24: 2550.
  • Heins A-L, Lundin L, Nunes I, Gernaey KV, Sørensen SJ, Lantz AE (2019): The effect of acetate on population heterogeneity in different cellular characteristics of Escherichia coli in aerobic batch cultures. Biotechnol Prog 35: e2796.
  • Riegler P, Bieringer E, Chrusciel T, Stärz M, Löwe H, Weuster Botz D (2019): Continuous conversion of CO2/H2 with Clostridium aceticum in biofilm reactors. Biores Technol, DOI 10.1016/j.biortech.2019.121760.
  • Najbauer EE Movellan KT, Schubeis T, Schwarzer T, Castiglione K, Giller K, Pintacuda G, Becker S, Andreas LB (2019): Probing membrane protein insertion into lipid bilayers by solid-state NMR. ChemPhysChem 20: 302 –310.
  • Wagner SG, Mähler C, Polte I, von Poschinger J, Löwe H, Kremling A, Pflüger-Grau K (2019): An automated and parallelised DIY-dosing unit for individual and complex feeding profiles: Construction, validation and applications. PLoS ONE 14: e0217268.
  • Schädler T, Caballero D, Oliveira L, Garbe D, Brück T, Weuster-Botz D (2019): Production of lipids with Microchloropsis salina in open thin-layer cascade photobioreactors. Biores Technol 289: 121682
  • Mähler C, Kratzl F, Vogel M, Vinnenberg S, Weuster-Botz D, Castiglione K (2019): Loop swapping as a potent approach to increase ene reductase activity with NADH. Adv Syn Cat 361: 2505-2513.
  • Burger C, Kessler C, Gruber S, Ehrenreich A, Liebl W, Weuster-Botz D (2019): L-erythrulose production with a multideletion strain of Gluconobacter oxydans. Appl Microbiol Biotechnol 103: 4393–4404.
  • Pfaffinger CE, Severin TS, Apel AC, Göbel J, Sauter J, Weuster-Botz D (2019): Light-dependent growth kinetics enable scale-up of well-mixed phototrophic bioprocesses in different types of photobioreactors. J Biotechnol 297: 41-48.
  • Nowotny P, Hermann J, Li J, Krautenbacher AE, Klöpfer K, Hekmat D, Weuster-Botz D (2019): Rational crystal contact engineering of Lactobacillus brevis alcohol dehydrogenase to promote technical protein crystallization. Cryst Growth Des 19: 2380–2387.
  • Severin T, Brück T, Weuster-Botz D (2019): Validated numerical fluid simulation of a thin-layer cascade photobioreactor in OpenFOAM. Eng Life Sci 19: 97-103.
  • Martinez A, Kuhn M, Briesen H, Hekmat D (2019) Enhancing the X-ray contrast of polymeric biochromatography particles for three-dimensional imaging, J Chrom A 1590: 65-72.
  • Riegler P, Chrusciel T, Mayer A, Doll K, Weuster Botz D (2019): Reversible retrofitting of a stirred-tank bioreactor for gas-lift operation to perform syngas fermentation studies. Biochem Eng J 141: 89-101.

Publikationen 2018

  • Hermann J, Nowotny P, Schrader TE, Biggel P, Hekmat D, Weuster-Botz D (2018): Neutron and X-ray crystal structures of Lactobacillus brevis alcohol dehydrogenase reveal new insights on hydrogen bonding pathways. Acta Cryst F74: 754-764.
  • Schwarzer TS, Klermund L, Wang G, Castiglione K (2018): Membrane functionalization of polymersomes: allevating mass transport limitations by integrating multiple selective membrane transporters for the diffusion of chemically diverse molecules. Nanotechnol 29: 44LT01.
  • Wiechert W, Scheper T, Weuster-Botz D (2018): Neue Schubkraft für die Biotechnologie: Miniaturisierung, Automatisierung und Digitalisiereung revolutionieren die Entwicklung biotechnologischer Prozesse und Produkte. Positionspapier DECHEMA Biotechnologie.
  • Tröndle J, Trachtmann N, Sprenger GA, Weuster-Botz D (2018): Production of L-tryptophan from glycerol using recombinant Escherichia coli. Biotechnol Bioeng 115: 2881–2892.
  • Mayer A, Schädler T, Trunz S, Stelzer T, Weuster-Botz D (2018): Carbon monoxide conversion with Clostridium aceticum. Biotechnol Bioeng 115: 2740–2750.
  • Weuster-Botz D, Takors R (2018): Wachstumskinetik. In Chmiel H, Takors R, Weuster-Botz D (Herausgeber): Bioprozesstechnik. 4. Auflage, Springer-Verlag GmbH, Berlin: 45-70.
  • Takors R, Weuster-Botz D (2018): Prozessmodelle. In Chmiel H, Takors R, Weuster-Botz D (Herausgeber): Bioprozesstechnik. 4. Auflage, Springer-Verlag GmbH, Berlin: 71-106.
  • Chmiel H, Weuster-Botz D (2018): Bioreaktoren. In Chmiel H, Takors R, Weuster-Botz D (Herausgeber): Bioprozesstechnik. 4. Auflage, Springer-Verlag GmbH, Berlin: 157-230.
  • Castiglione K, Weuster-Botz D (2018): Enzymatische Prozesse. In Chmiel H, Takors R, Weuster-Botz D (Herausgeber): Bioprozesstechnik. 4. Auflage, Springer-Verlag GmbH, Berlin: 403-448.
  • Doll K, Rückel A, Kämpf P, Weuster-Botz D (2018): Two stirred-tank bioreactors in series enable continuous production of alcohols from carbon monoxide with Clostridium carboxidivorans. Bioproc Biosys Eng 41:1403-1416.
  • Klermund L, Castiglione K (2018): Polymersomes as nanoreactors for preparative biocatalytic applications: current challenges and future perspectives. Bioproc Biosys Eng 41:1233–1246.
  • Takors R, Kopf M, Mampel J, Bluemke W, Blombach B, Eikmanns B, Bengelsdorf F, Weuster-Botz D, Dürre P (2018): Using gas mixtures of CO, CO2, and H2 as microbial substrates: The dos and don’ts of successful technology transfer from lab to production scale. Microb Biotechnol 11: 606-625.
  • Al-Rowaihi I, Kick B, Grötzinger SW, Burger C, Karan R, Weuster-Botz D, Eppinger J, Arold ST (2018): A two-stage biological gas to liquid transfer process to convert carbon dioxide into bioplastic. Biores Technol Report 1: 61–68.
  • Bengelsdorf FR, Beck MH, Erz C, Hoffmeister S, Karl MH, Riegler P, Wirth S, Poehlein A, Weuster-Botz D, Dürre P (2018): Bacterial anaerobic synthesis gas (syngas) and CO2 + H2 fermentation. Adv Appl Microbiol 103: 143-221.
  • Heins AL, Weuster-Botz D (2018): Population heterogeneity in bioprocesses – the diversity of single cells. Bioproc Biosys Eng 41: 889-916.
  • Severin TS, Apel A, Brück T, Weuster-Botz D (2018): Investigation of vertical mixing in open thin-layer cascade reactors using Computational Fluid Dynamics. Chem Eng Res Design 132: 436-444.
  • Tröndle J, Albermann C, Weiner M, Sprenger GA, Weuster-Botz D (2018): Phosphoenolpyruvate transporter enables targeted perturbation during metabolic analysis of L-phenylalanine production with Escherichia coli. Biotechnol J 13: 1700611.
  • Poschenrieder ST, Schiebel SK, Castiglione K (2018): Stability of polymersomes with focus on their use as nanoreactors. Eng Life Sci 18: 101-113.
  • Poschenrieder ST, Hanzlik M, Castiglione K (2018): Polymersome formation mechanism and formation rate in stirred-tank reactors. J Appl Polym Sci 135: 46077.
  • Priebe X, Daugulis AJ (2018): Thermodynamic affinity-based considerations for the rational selection of biphasic systems for microbial flavor and fragrance production. J Chem Technol Biotechnol 93: 656-666.
  • Schmidt S, Castiglione K, Kourist R (2018): Overcoming the incompatibility challenge in chemoenzymatic and multi-catalytic cascade reactions. Chemistry – A European Journal 24: 1755-1768.
  • Priebe X, Daschner M, Schwab W, Weuster-Botz D (2018): Rational selection of biphasic reaction systems for geranyl glucoside production by Escherichia coli whole-cell biocatalysts. Enzyme Microb Technol 112: 79-87
  • Koller A, Wolf L, Brück T, Weuster-Botz D (2018): Studies on the scale-up of biomass production with Scenedesmus sp. in flat-plate gas-lift photobioreactors. Bioproc Biosys Eng 41: 213–220.
  • Groetzinger SW, Karan R, Strillinger E, Bader S, Frank A, Al Rowaihi I, Akal A, Wackerow W, Archer JA, Rueping M, Weuster-Botz D, Groll, M, Eppinger J, Arold ST (2018): Identification and experimental characterization of an extremophilic brine pool alcohol dehydrogenase from single amplified genomes. ACS Chem Biol 13: 161-170.

Publikationen 2017

  • Praetorius F, Kick B, Behler KL, Honemann MN, Weuster-Botz D, Dietz H (2017): Biotechnological mass production of DNA origami. Nature 552: 84-87.
  • Schmidt S, Castiglione K, Kourist R (2017): Overcoming the Incompatibility Challenge in Chemoenzymatic and Multi-Catalytic Cascade Reactions. Chem Eur J 23: 1-15.
  • Mayer A, Weuster-Botz D (2017): Reaction engineering analysis of the autotrophic energy metabolism of Clostridium aceticum. FEMS Microbiol Lett 364: fnx219.
  • Mairinger T, Tröndle J, Hanscho M, Hann S (2017): On-line clean-up and LC-MS analysis of primary metabolites in cell culture supernatants. Anal Methods 9: 5703-5710.
  • Dorn M, Eschbach F, Hekmat D, Weuster-Botz D (2017): Influence of different packing methods on the hydrodynamic stability of chromatography columns. J Chromatogr A 1516: 89−101.
  • Dorn M, Schilde C, Burmeister CF, Hekmat D (2017): Mechanical characterization of compressible chromatographic particles. Powder Technol 320: 213−222.
  • Weiner M, Tröndle J, Albermann C, Sprenger GA, Weuster-Botz D (2017): Metabolic control analysis of L-phenylalanine production from glycerol with engineered E. coli using data from short-term steady-state perturbation experiments. Biochem Eng J 126: 86–100.
  • Apel AC, Pfaffinger CE, Basedahl N, Mittwollen N, Göbel J, Sauter J, Brück T, Weuster-Botz D (2017): Open thin-layer cascade reactors for saline microalgae production evaluated in a physically simulated Mediterranean summer climate. Algal Research 25 (2017): 381–390.
  • Hekmat D, Huber M, Lohse C, von den Eichen N, Weuster-Botz D (2017): Continuous crystallization of proteins in a stirred classified product removal tank with a tubular reactor in bypass. Cryst Growth Des 17: 4162−4169.
  • Kick B, Behler KL, Severin TS, Weuster-Botz D (2017): Chemostat studies of bacteriophage M13 infected Escherichia coli JM 109 for continuous ssDNA production. J Biotechnol 258: 92-100.
  • Poschenrieder ST, Klermund L, Langer B, Castiglione K (2017): Determination of Permeability Coefficients of Polymersomal Membranes for Hydrophilic Molecules. Langmuir 33: 6011-6020.
  • Jain MG, Lalli D, Stanek J, Gowda C, Prakash S, Schwarzer TS, Schubeis T, Castiglione K, Andreas LB, Madhu PK, Pintacuda G, Agarwal V (2017): Selective 1H–1H Distance Restraints in Fully Protonated Proteins by Very Fast Magic-Angle Spinning Solid-State NMR. J Phys Chem Lett 8: 2399-2405.
  • Klermund L, Poschenrieder TS, Castiglione K (2017): Biocatalysis in Polymersomes: Improving Multienzyme Cascades with Incompatible Reaction Steps by Compartmentalization. ACS Catal 7: 3900-3904.
  • Severin TS, Plamauer S, Apel AC, Brück T, Weuster-Botz D (2017): Rapid salinity measurements for fluid flow characterisation using minimal invasive sensors. Chem Eng Sci 166: 161-167.
  • Hintermayer S, Weuster-Botz D (2017): Experimental validation of in silico estimated biomass yields of Pseudomonas putida KT2440. Biotechnol J 12:1600720.
  • Schmideder A, Weuster-Botz D (2017): High-performance recombinant protein production with Escherichia coli in continuously operated cascades of stirred-tank reactors. J Ind Microbiol Biotechnol 44: 1021–1029.
  • Schwarzer TS, Hermann M, Krishnan S, Simmel FC, Castiglione K (2017): Preparative refolding of small monomeric outer membrane proteins. Prot Expres Purif 132 (2017): 171-181.
  • Schmideder A, Schottroff F, Klermund L, Castiglione K, Weuster-Botz D (2017): Studies on the enzymatic synthesis of N-acetylneuraminic acid with continuously operated enzyme membrane reactors on a milliliter scale. Biochem Eng J 119: 9 - 19.
  • Koller AP, Wolf L, Weuster-Botz D (2017): Reaction engineering analysis of Scenedesmus ovalternus in a flat-plate gas-lift photobioreactor. Biores Technol 225: 165–174.
  • Meo A, Priebe X, Weuster-Botz D (2017): Lipid production with Trichosporon oleaginosus in a membrane bioreactor using microalgae hydrolysate. J Biotechnol 241: 1-10.
  • Kick B, Hensler S, Praetorius F, Dietz H, Weuster-Botz D (2017): Specific growth rate and multiplicity of infection affect high-cell density fermentation with bacteriophage M13 for ssDNA production. Biotechnol Bioeng 114: 777–784
  • Koller A, Löwe H, Schmid V, Mundt S, Weuster-Botz D (2017) Model-supported phototrophic growth studies with Scenedesmus obtusiusculus in a flat-plate photobioreactor. Biotechnol Bioeng 114: 308–320
  • Castiglione K, Fu Y, Polte I, Leupold S, Meo A, Weuster-Botz D (2017): Asymmetric whole-cell bioreduction of (R)-carvone by recombinant Escherichia coli with in situ substrate supply and product removal. Biochem Eng J 117: 102-111.
  • Poschenrieder ST, Schiebel SK, Castiglione K (2017): Polymersomes for biotechnological applications: Large-scale production of nano-scale vesicles. Eng Life Sci 17: 58–70.

Publikationen 2016

  • Pfaffinger CE, Schöne D, Trunz S, Löwe H, Weuster-Botz D (2016): Model-based optimization of microalgae areal productivity in flat-plate gas-lift photobioreactors. Algal Res 20: 153–163.
  • Schmideder A, Cremer JH, Weuster-Botz D (2016): Parallel steady state studies on a milliliter scale accelerate fed-batch bioprocess design for recombinant protein production with Escherichia coli. Biotechnology Progress 32: 1426–1435.
  • Hintermayer S, Yu S, Kroemer JO, Weuster-Botz D (2016): Anodic respiration of Pseudomonas putida KT2440 in a stirred-tank bioreactor. Biochem Eng J 115: 1-13.
  • Klermund L, Poschenrieder ST, Castiglione K (2016): Simple surface functionalization of polymersomes using non-antibacterial peptide anchors. J Nanobiotechnol 14:48.
  • Groher A, Weuster-Botz D (2016): General medium for the autotrophic cultivation of acetogens.
    Bioproc Biosys Eng 39: 1645-1650.
  • Schmideder A, Priebe X, Rubenbauer M, Hoffmann T, Huang F-C, Schwab W, Weuster-Botz D (2016): Non-water miscible ionic liquid improves biocatalytic production of geranyl glucoside with Escherichia coli overexpressing a glucosyltransferase. Bioproc Biosys Eng 39: 1409-1414.
  • Groher A, Weuster-Botz D (2016): Comparative reaction engineering analysis of different acetogenic bacteria for gas fermentation. J Biotechnol 228: 82-94.
  • Klermund L, Riederer A, Hunger A, Castiglione K (2016): Protein engineering of a bacterial N-acyl-D-glucosamine 2-epimerase for improved stability under process conditions. Enzyme Microb Technol 87-88: 70-78.
  • Kantzow C, Weuster-Botz D (2016): Effects of hydrogen partial pressure on autotrophic growth and product formation of Acetobacterium woodii. Bioproc Biosys Eng 39: 1325–1330.
  • Poschenrieder ST, Wagner SG, Castiglione K (2016): Efficient production of uniform nanometer-sized polymer vesicles in stirred-tank reactors. J Appl Polym Sci 133: 43274.
  • Dorn M, Hekmat D (2016): Simulation of the dynamic packing behavior of preparative chromatography columns via discrete particle modeling. Biotechnol Prog 32: 363-371.
  • Schmideder A, Hensler S, Lang M, Stratmann A, Giesecke U, Weuster-Botz D (2016): High-cell-density cultivation and recombinant protein production with Komagataella pastoris in stirred-tank bioreactors from milliliter to cubic meter scale. Proc Biochem 451: 177-184.
  • Strillinger E, Grötzinger SW, Allers T, Groll M, Eppinger J, Weuster-Botz D (2016): Production of halophilic proteins with Haloferax volcanii H1895 in a stirred tank bioreactor. App Microbiol Biotechnol 100: 1183–1195.
  • Weiner M, Tröndle J, Albermann C, Sprenger GA, Weuster-Botz D (2016): Perturbation experiments: Approaches for metabolic pathway analysis in bioreactors. Adv Biochem Eng Biotechnol 152: 91-136.

Publikationen 2015

  • Sührer I, Langemann T, Lubitz W, Weuster-Botz D, Castiglione K (2015): A novel one-step expression and immobilization method for the production of biocatalytic preparations. Microbial Cell Factories 14: 180-189. 
  • Kantzow C, Mayer A, Weuster-Botz D (2015): Continuous gas fermentation by Acetobacterium woodii in a submerged membrane reactor with full cell retention. J Biotechnol 212: 11-18.
  • Schmideder A, Severin TS, Cremer J, Weuster-Botz D (2015): A novel milliliter-scale chemostat system for parallel cultivation of microorganisms in stirred-tank bioreactors. J Biotechnol 210: 19-24.
  • Faust G, Stand A, Weuster-Botz D (2015): IPTG can replace lactose in auto-induction media to enhance protein expression in batch-cultured Escherichia coli. Eng Life Sci 15: 824-829.
  • Kick B, Praetorius F, Dietz H, Weuster-Botz D (2015): Efficient production of single-stranded phage DNA as scaffolds for DNA origami. Nano Letters 15: 4672-4676.
  • Hekmat D, Breitschwerdt P, Weuster-Botz D (2015): Purification of proteins from solutions containing residual host cell proteins via preparative crystallization. Biotechnol Lett 37:1791-1801.
  • Janzen N, Schmidt M, Krause C, Weuster-Botz D (2015): Evaluation of fluorimetric pH-sensors for bioprocess monitoring at low pH. Bioproc Biosys Eng 38: 1685-1692.
  • Klermund L, Riederer A, Groher A, Castiglione K (2015): High-level soluble expression of a bacterial N-acyl-D-glucosamine 2-epimerase in recombinant Escherichia coli. Protein Expr Purif 111: 36-41.
  • Weiner M, Tröndle J, Schmideder A, Binder K, Albermann C, Sprenger GA, Weuster-Botz D (2015): Parallelized small-scale production of uniformly 13C-labeled cell-extract for quantitative metabolome analysis, Anal Biochem 478: 134-140.
  • Hekmat D (2015): Large-scale crystallization of proteins for purification and formulation. Bioproc Biosys Eng 38:1209–1231.
  • Apel A, Weuster-Botz D (2015): Engineering solutions for open microalgae mass cultivation and realistic indoor simulation of outdoor environments. Bioproc Biosys Eng 38: 995-1008.
  • Sun B, Hartl F, Castiglione K, Weuster-Botz D (2015): Dynamic mechanistic modeling of the multienzymatic one-pot reduction of dehydrocholic acid to 12-keto ursodeoxycholic acid with competing substrates and cofactors. Biotechnol Prog 31: 375–386.
  • Fu Y, Castiglione K, Weuster-Botz D (2015): Ene-reductases from cyanobacteria for industrial biocatalysis. Industrial Biocatalysis (Ed. Grunwald P), Pan Stanford Publishing Pte. Ltd., 631-661.
  • Hekmat D, Maslak D, Freiherr von Roman M, Breitschwerdt P, Ströhle C, Vogt A, Berensmeier S, Weuster-Botz D (2015): Non-chromatographic preparative purification of enhanced green fluorescent protein. J Biotechnol 194: 84-90.

Publikationen 2014

  • Albermann C, Weiner M, Tröndle J, Weuster-Botz D, Sprenger GA (2014): Utilization of organophosphate:phosphate antitransporter for isotope-labeling experiments in  E. coli. FEMS Microbiol Lett 361: 52–61.
  • Faust G, Janzen N, Bendig C, Römer L, Kaufmann K, Weuster-Botz D (2014): Feeding strategies enhance high cell density cultivation and protein expression in milliliter-scale bioreactors. Biotechnol J 9: 1293-1303.
  • Weiner M, Tröndle J, Albermann C, Sprenger GA, Weuster-Botz D (2014): Carbon storage in recombinant Escherichia coli during growth on glycerol and lactic acid. Biotechnol Bioeng 111: 2508-2519.
  • Sührer I, Haslbeck M, Castiglione K (2014): Asymmetric synthesis of a Fluoxetin precursor with an artificial fusion protein of a ketoreductase and a formate dehydrogenase. Proc Biochem 49: 1527-1532.
  • Straub M, Demler M, Weuster-Botz D, Dürre P (2014): Selective enhancement of autotrophic actetate production with genetically modified Acetobacterium woodii. J Biotechnol 178: 67-72.
  • Weiner M, Tröndle J, Albermann C, Sprenger GA, Weuster-Botz D (2014): Improvement of constraint-based flux estimation during L-phenylalanine production with Escherichia coli using targeted knock-out mutants. Biotechnol Bioeng 111: 1406-1416.
  • Weiner M, Albermann C, Gottlieb K, Sprenger GA, Weuster-Botz D (2014): Fed-batch production of L-phenylalanine from glycerol and ammonia with recombinant Escherichia coli. Biochem Eng J 83: 62-69.
  • Brüning S, Weuster-Botz D (2014): CFD analysis of interphase mass transfer and energy dissipation in a milliliter-scale stirred-tank reactor for filamentous microorganisms. Chem Eng Res Des 92: 240-248.

Publikationen 2013

  • Klermund L, Groher A, Castiglione K (2013): New N-acyl-D-glucosamine 2-epimerases from cyanobacteria with high activity in the absence of ATP and low inhibition by pyruvate. J Biotechnol 168: 256-263.
  • Hekmat D, Kuhn M, Meinhardt V, Weuster-Botz D (2013): Modeling of transient flow through a viscoelastic preparative chromatography packing. Biotechnol Prog 29: 958-967.
  • Hebel D, Huber S, Stanislawski B, Hekmat D (2013): Stirred batch crystallization of a therapeutic antibody fragment. J Biotechnol 166: 206-211.
  • Hebel D, Ürdingen M, Hekmat D, Weuster-Botz D (2013): Development and scale-up of high-yield crystallization processes of lysozyme and lipase using additives. Cryst Growth Des 13: 2499-2506.
  • Smejkal B, Agrawal NJ, Helk B, Schulz H, Giffard M, Mechelke M, Ortner F, Heckmeier P, Trout BL, Hekmat D (2013): Fast and scalable purification of a therapeutic full-length antibody based on process crystallization. Biotechnol Bioeng 110: 2452-2461.
  • Smejkal B, Helk B, Rondeau JM, Anton S, Wilke A, Scheyerer P, Fries J, Hekmat D, Weuster-Botz D (2013): Protein crystallization in stirred systems – Scale-up via the maximum local energy dissipation. Biotechnol Bioeng 110: 1956-1963.
  • Riedlberger P, Brüning S, Weuster-Botz D (2013): Characterization of stirrers for screening studies of enzymatic biomass hydrolyses on a milliliter-scale. Bioproc Biosys Eng 36: 927-935.
  • Liu L, Braun M, Gebhardt G, Weuster-Botz D, Gross R, Schmid RD (2013): One step synthesis of 12-ketoursodeoxycholic acid from dehydrocholic acid using a multienzymatic system. Appl Microbiol Biotechnol 97: 633-639.
  • Bendig C, Weuster-Botz D (2013): Reaction engineering analysis of cellulase production with Trichoderma reesei RUT-C30 with intermittent substrate supply. Bioproc Biosys Eng 36: 893-900.
  • Hölsch K, Sührer I, Heusel M, Weuster-Botz D (2013): Engineering of formate dehydrogenase: Synergistic effect of mutations affecting cofactor specificity and chemical stability. Appl Microbiol Biotechnol 97: 2473-2481.
  • Fu Y, Castiglione K, Weuster-Botz D (2013): Comparative characterization of novel ene-reductases from cyanobacteria. Biotechnol Bioeng 110: 1293-1301.
  • Delhomme C, Schaper LA, Zhang-Preße M, Raudaschl-Sieber G, Weuster-Botz D, Kühn FE (2013): Catalytic hydrogenation of levulinic acid in aqueous phase. J Organomet Chem 724: 297-299.
  • Sun B, Kantzow C, Bresch S, Castiglione K, Weuster-Botz D (2013): Multi-enzymatic one-pot reduction of dehydrocholic acid to 12-keto-ursodeoxycholic acid with whole-cell biocatalysts. Biotechnol Bioeng 110: 68-77.

Publikationen 2012

  • Anselment B, Schoemig V, Kesten C, Weuster-Botz D (2012): Statistical versus stochastic experimental design – an experimental comparison on the example of protein refolding. Biotechnol Prog 28: 1499-1506.
  • Fu Y, Hölsch K, Weuster-Botz D (2012): A novel ene-reductase from Synechococcus sp. PCC 7942 for the asymmetric reduction of alkenes. Proc Biochem 47: 1988–1997.
  • Dennewald D, Weuster-Botz D (2012): Ionic liquids and whole-cell catalyzed processes. In Dominguez de Maria P (ed): Ionic liquids in biotransformations and organocatalysis: Solvents and beyond, John Wiley & Sons, Inc. Hoboken, New Jersey: 261-314.
  • Asano Y, Hölsch K (2012): Isomerizations. In Drauz K, Gröger H, May O (ed.): Enzyme Catalysis in Organic Synthesis, Third Edition, Vol. 3. Wiley-VCH,  Weinheim: 1609-1684.
  • Groher A, Hölsch K (2012): Mechanistic model for the synthesis of N-acetylneuraminic acid using N-acetylneuraminate lyase from Escherichia coli. J Mol Cat B Enz 83: 1-7.
  • Höfel T, Faust G, Reinecke L, Rudinger N, Weuster-Botz D (2012): Comparative reaction engineering studies for succinic acid production from sucrose by metabolically engineered Escherichia coli in fed-batch operated stirred tank bioreactors. Biotechnol J 7: 1277-1287.
  • Braun M, Sun B, Anselment B, Weuster-Botz D (2012): Novel whole-cell biocatalysis with recombinant hydroxysteroid dehydrogenases for the asymmetric reduction of dehydrocholic acid. Appl Microbiol Biotechnol 95: 1457-1468.
  • Delhomme C, Goh S, Kühn F, Weuster-Botz D (2012): Esterification of bio-based succinic acid in biphasic systems: Comparison of chemical and biological catalysts. J Mol Cat B Enz 80: 39-47.
  • Schmidt M, Weuster-Botz D (2012): Reaction engineering studies of acetone-butanol-ethanol fermentation with Clostridium acetobutylicum. Biotechnol J 7: 656-661.
  • Lehmann D, Hönicke D, Ehrenreich A, Schmidt M, Weuster-Botz D, Bahl H, Lütke-Eversloh T (2012): Modifying the product pattern of Clostridium acetobutylicum: Physiological effects of disrupting the acetate and acetone formation pathways. Appl Microbiol Biotechnol 94: 743-754.
  • Riedlberger P, Weuster-Botz D (2012): New miniature stirred-tank bioreactors for parallel study of enzymatic biomass hydrolysis. Bioresource Technol 106: 138-146.
  • Dennewald D, Hortsch R, Weuster-Botz D (2012): Evaluation of parallel millilitre-scale stirred-tank bioreactors for the study of biphasic whole-cell biocatalysis with ionic liquids. J Biotechnol 157: 253-257.

Publikationen 2011

  • Kurzrock T, Schallinger S, Weuster-Botz D (2011): Integrated separation process for isolation and purification of bio-succinic acid. Biotechnol Prog 27: 1623-1628.
  • Link H, Weuster-Botz D (2011): Upstream Processing: Medium Formulation and Development. In Murray Moo-Young (ed.): Comprehensive Biotechnology, Second Edition, Vol. 2. Elsevier: 119-134.
  • Braun M, Link H, Liu L, Schmid RD, Weuster-Botz D (2011): Biocatalytic process optimization based on mechanistic modeling of cholic acid oxidation with cofactor regeneration. Biotechnol Bioeng 108: 1307-1317.
  • Hofinger M, Berthold G, Weuster-Botz D (2011): Microbial production of homogenously layered cellulose pellicles in a membrane bioreactor. Biotechnol Bioeng 108: 2237-2240.
  • Kurzrock T, Weuster-Botz D (2011): New reactive extraction systems for separation of bio-succinic acid. Bioproc Biosys Eng 34: 779-787.
  • Gebhardt G, Hortsch R, Kaufmann K, Arnold M, Weuster-Botz D (2011): A new microfluidic concept for parallel operated millilitre-scale stirred-tank bioreactors. Biotechnol Prog 27: 684-690.
  • Dennewald D, Pitner WR, Weuster-Botz D (2011): Recycling of the ionic liquid phase in process integrated biphasic whole-cell biocatalysis. Proc Biochem 46: 1132-1137.
  • Maslak D, Weuster-Botz D (2011): Combination of hydrodynamic cavitation with chlorine dioxide for disinfection of water. Eng Life Sci 11: 350-358.
  • Hölsch K, Weuster-Botz D (2011): Kinetic mechanism of 3-ketoacyl-(acyl-carrier-protein) reductase from Synechococcus sp. strain PCC 7942: A useful enzyme for the production of chiral alcohols. J Mol Cat B: Enz 69: 89-94.
  • Hortsch R, Weuster-Botz D (2011): Growth and recombinant protein expression with Escherichia coli in different batch cultivation media. Appl Microbiol Biotechnol 90: 69-76.
  • Hekmat D, Mornhinweg R, Bloch G, Sun Y, Jeanty P, Neubert M, Weuster-Botz D (2011): Macroscopic investigation of the transient hydrodynamic memory behavior of preparative packed chromatography beds. Journal of Chromatography A 1218: 944-950.
  • Hortsch R, Krispin H, Weuster-Botz D (2011): Process performance of parallel bioreactors for batch cultivation of Streptomyces tendaeBioproc Biosys Eng34: 297-304.
  • Demler M, Weuster-Botz D (2011): Reaction engineering analysis of hydrogenotrophic production of acetic acid by Acetobacterium woodiiBiotechnol Bioeng 108: 470-474.

Publikationen 2010

  • Raab A, Gebhardt G, Bolotina N, Weuster-Botz D, Lang C (2010): Metabolic engineering of Saccharomyces cerevisiae for the biotechnological production of succinic acid. Metabol Eng12: 518-525.
  • Anselment B, Baerend D, Mey E, Buchner J, Weuster-Botz D, Haslbeck M (2010): Experimental optimization of protein refolding with a genetic algorithm. Protein Science 19: 2085-2095.
  • Hortsch R, Weuster-Botz D (2010): Milliliter-scale stirred tank reactors for the cultivation of microorganisms. Adv Appl Microbiol 73: 61-82.
  • Hölsch K, Weuster-Botz D (2010): Enantioselective reduction of prochiral ketones by engineered bifunctional fusion proteins. Biotechnol Appl Biochem 56: 131-140.
  • Hölsch K, Weuster-Botz D (2010): New oxidoreductases from cyanobacteria: exploring nature’s diversity. Enz Microbial Technol 47: 228-235.
  • Höfel T, Wittmann E, Reinecke L, Weuster-Botz D (2010): Reaction engineering studies for the production of 2-hydroxyisobutyric acid with recombinant Cupriavidus necator H16. Appl Microbiol Biotechnol 88: 477-484.
  • Hortsch R, Stratmann A, Weuster-Botz D (2010): New milliliter-scale stirred tank bioreactors for the cultivation of mycelium forming microorganisms. Biotechnol Bioeng 106: 443-451. 
  • Hortsch R, Weuster-Botz D (2010): Power consumption and maximum energy dissipation in a milliliter-scale bioreactor. Biotechnol Prog 26: 595-599.
  • Kurzrock T, Weuster-Botz D (2010): Recovery of succinic acid from fermentation broth. Biotechnol Lett 32: 331-339.
  • Link H, Anselment B, Weuster-Botz D (2010): Rapid media transition: An experimental approach for steady state analysis of metabolic pathways. Biotechnol Prog 26: 1-10.

Publikationen 2009

  • Bräutigam S, Dennewald D, Schürmann M, Lutje-Spelberg J, Pitner W-R, Weuster-Botz D (2009): Whole cell biocatalysis: Evaluation of new hydrophobic ionic liquids for efficient asymmetric reduction of prochiral ketones. Enz Microbial Technol 45: 310-316. 
  • Vester A, Hans M, Hohmann H-P, Weuster-Botz D (2009): Discrimination of riboflavin producing Bacillus subtilis strains based on their fed-batch process performances on a millilitre scale. Appl Microbiol Biotechnol 84: 71-76.
  • Gadler P, Reiter T, Hölsch K, Weuster-Botz D, Faber K (2009): Enantiocomplementary inverting sec-alkylsulfatase activity in cyano- and thio-bacteria Synechococcus and Paracoccus spp.: selectivity enhancement by medium engineering. Tetrahedron: Asymmetry 20: 115-118. 
  • Delhomme C, Weuster-Botz D, Kühn FE (2009): Succinic acid from renewable resources as a C4 building-block chemical - a review of the catalytic possibilities in aqueous media. Green Chem 11 (2009): 13-26.

Publikationen 2008

  • Hölsch K, Havel J, Haslbeck M, Weuster-Botz D (2008): Identification, cloning and characterization of a novel ketoreductase from the cyanobacterium Synechococcus sp. strain PCC 7942. Appl Environ Microbiol 74: 6697-6702.
  • Knepper A, Schleicher M, Klauke M, Weuster-Botz D (2008): Enhancement of the NAD(P)(H) pool in Saccharomyces cerevisiaeEng Life Sci  8: 381-389.
  • Link H, Anselment B, Weuster-Botz D (2008): Leakage of adenylates during cold methanol/glycerol quenching of Escherichia coliMetabolomics 4: 240-247. 
  • Link H, Vera J, Weuster-Botz D, Torres Darias N, Franco-Lara E (2008): Multi-objective steady state optimization of biochemical reaction networks using a constrained genetic algorithm. Comp Chem Eng 32: 1707-1713.
  • Havel J, Franco-Lara E, Weuster-Botz D (2008): A parallel bubble column system for the cultivation of phototrophic microorganisms. Biotechnol Lett 30: 1197-1200.
  • Hekmat D, Hebel D, Weuster-Botz D (2008): Crystalline proteins as an alternative to standard formulations. Chem Eng Technol 31: 911-916.
  • Kusterer A, Krause C, Kaufmann K, Arnold M, Weuster-Botz D (2008): Fully automated single-use stirred-tank bioreactors for parallel microbial cultivations. Bioprocess Biosyst Eng 31: 207-215.

Publikationen 2007

  • Weuster-Botz D (2007): Process intensification of whole-cell biocatalysis with ionic liquids.The Chemical Record 7: 334-340.
  • Hekmat D, Hebel D, Schmid H, Weuster-Botz D (2007): Crystallization of lysozyme: from vapor diffusion experiments to batch crystallization in agitated ml-scale vessels. Proc Biochem 42: 1649-1654. 
  • Link H, Weuster-Botz D (2007): Steady-state analysis of metabolic pathways: Comparing the double modulation method and the lin-log approach. Metab Eng 9: 433-441.
  • Hekmat D, Hebel D, Joswig S, Schmidt M, Weuster-Botz D (2007): Advanced protein crystallization using water-soluble ionic liquids as crystallization additives. Biotechnol Lett  29: 1703-1711.
  • Bräutigam S, Bringer-Meyer S, Weuster-Botz D (2007): Asymmetric whole cell biotransformations in biphasic ionic liquid/water-systems by use of recombinant Escherichia coli with intracellular cofactor regeneration. Tetrahedron: Asymmetry 18: 1883-1887. 
  • Tiemeyer A, Link H, Weuster-Botz D (2007): Kinetic studies on autohydrogenotrophic growth of Ralstonia eutropha with nitrate as terminal electron acceptor. Appl Microbiol Biotechnol  76: 75-81.
  • Hiller J, Franco-Lara E, Papaioannou V, Weuster-Botz D (2007): Fast sampling and quenching procedures for microbial metabolic profiling. Biotechnol Lett  29: 1161-1167.
  • Hiller J, Franco-Lara E, Weuster-Botz D (2007): Metabolic profiling of Escherichia coli cultivations: evaluation of extraction and metabolite analysis procedures. Biotechnol Lett  29: 1169-1178.
  • Havel J, Weuster-Botz D (2007): Cofactor regeneration in phototrophic cyanobacteria applied for asymmetric reduction of ketones. Appl Microbiol Biotechnol 75: 1031-1037. 
  • Franco-Lara E, Weuster-Botz D (2007): Application of fuzzy-logic models for metabolic control analysis. J Theoretic Biol 245: 391-399.
  • Weuster-Botz D, Hekmat D, Puskeiler R, Franco-Lara E (2007): Enabling Technologies: Fermentation and Downstream Processing. Adv Biochem Eng Biotechnol 105: 205-247.
  • Knorr B, Schlieker H, Hohmann H-P, Weuster-Botz D (2007): Scale-down and parallel operation of the riboflavin production process with Bacillus subtilisBiochem Eng J33: 263-274. 

Publikationen 2006

  • Link H, Weuster-Botz D (2006): Genetic algorithm for multi-objective experimental optimization. Bioprocess Biosyst Eng 29: 385-390.
  • Franco-Lara E, Havel J, Peterat F, Weuster-Botz D (2006): Model-supported optimization of phototrophic growth in a stirred-tank photo-bioreactor. Biotechnol Bioeng 95: 1177-1187.
  • Franco-Lara E, Link H, Weuster-Botz D (2006): Evaluation of artificial neural networks for modelling and optimization of medium composition with a genetic algorithm. Proc Biochem 41: 2200-2206. 
  • Havel J, Link H, Hofinger M, Franco-Lara E, Weuster-Botz D (2006): Comparison of genetic algorithms for experimental multi-objective optimization on the example of medium design for cyanobacteria. Biotechnol J 1: 549-555.
  • Havel J, Weuster-Botz D (2006): Comparative study of cyanobacteria as biocatalysts for the asymmetric synthesis of chiral building blocks. Eng Life Sci 6: 175-179.
  • Pfruender H, Jones R, Weuster-Botz D (2006): Water immiscible ionic liquids as solvents for whole cell biocatalysis. J Biotechnol 124: 182-190.
  • Engelking H, Pfaller R, Wich G, Weuster-Botz D (2006): Reaction engineering studies on ß-ketoester reductions with whole cells of recombinant Saccharomyces cerevisiaeEnz Microb Technol 38: 536-544.

Publikationen 2005

  • Puskeiler R, Weuster-Botz D (2005): Combined sulfite method for the measurement of the oxygen transfer coefficient kLa in bioreactors. J Biotechnol 120: 430-438.
  • Weuster-Botz D, Puskeiler R, Kusterer A, Kaufmann K, John GT, Arnold M (2005): Methods and milliliter scale devices for high-throughput bioprocess design. Bioprocess Biosyst Eng 28: 109-119
  • Franco-Lara E, Weuster-Botz D (2005): Estimation of optimal feeding strategies for fed-batch bioprocesses. Bioproc Biosys Eng 27: 255-262.
  • Amidjojo A, Franco-Lara E, Nowak A, Link H, Weuster-Botz D (2005): Asymmetric synthesis of tert-butyl (3R, 5S) 6-chlorodihydroxyhexanoate with Lactobacillus kefir. Appl Microbiol Biotechnol 69: 9-15.
  • Puskeiler R, Kusterer A, John GT, Weuster-Botz D (2005): Miniature bioreactors for automated high-throughput bioprocess design (HTBD): reproducibility of parallel fed-batch cultivations with Escherichia coliBiotechnol Appl Biochem 42: 227-235.
  • Pfruender H, Amidjojo M, Hang F, Weuster-Botz D (2005): Production of Lactobacillus kefir cells for asymmetric synthesis of a 3,5-dihydroxycarboxylate. Appl Microbiol Biotechnol 67: 619-622. 
  • Puskeiler R, Kaufmann K, Weuster-Botz D (2005): Development, parallelization, and automation of a gas-inducing milliliter-scale bioreactor for high-throughput bioprocess design (HTBD). Biotechnol Bioeng 89: 512-523.
  • Amidjojo M, Weuster-Botz D (2005): Asymmetric synthesis of the chiral synthon ethyl (S)-4-chloro-3-hydroxybutanoate using Lactobacillus kefirTetrahedron: Asymmetry 16: 899-901.
  • Weuster-Botz D (2005): Parallel reactor systems for the bioprocess development. In Scheper T (ed): Advances in BiochemicalEngineering/Biotechnology 92, Springer-Verlag Berlin Heidelberg New York: 125-143